Atorvastatin Ameliorates Urografin Induced Nephropathy in the Presence of Dehydration as a Risk Factor: A Histological and Morphometric Study on a Rat Model

Hassem, Amal; Rafla, Moheb; Mahmoud, Hoda Mohammed; El-Nefiawy, Nagwa Ibrahim; Shoukry, Youssef; Al Neklawy, Ahmed Farid

doi:10.21608/ejana.2020.21238.1056

Atorvastatin Ameliorates Urografin Induced Nephropathy in the Presence of Dehydration as a Risk Factor: A Histological and Morphometric Study on a Rat Model

Document Type : Original Article

Authors

¹ Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University.

² department of anatomy , faculty of medicine , ain shams university

³ Anatomy and Embryology Department, Faculty of Medicine, Ain Shams University, Cairo, Egypt. Department of Physiological Sciences, Fakeeh College for Medical Sciences, Jeddah, Kingdom of Saudi Arabia.

10.21608/ejana.2020.21238.1056

Abstract

In this study, the protective role of atorvastatin on urografin induced renal injury with the presence of dehydration as a risk factor was assessed. Forty two male adult albino rats were randomly divided into seven groups (6 rats / group). Group I (control), group II (atorvastatin), group III (dehydration), group IV (urografin), group V (dehydration with urografin), group VI (atorvastatin pretreatment), and group VII (recovery). At the end of the experiment for each group, blood samples were collected for estimation of serum level of interleukin-6 and creatinine. After sacrifice, kidneys were extracted and processed for light and transmission electron microscopy. Examination of kidney sections from both group III and group IV showed an apparent increase in the radius of proximal and distal convoluted tubules. Also hyaline casts, vacuolated tubular cells, and vascular congestion were seen. Moreover, there was increase in serum levels of both interleukin-6 and creatinine. All these findings were found in group V. In addition, there was an apparent increase in the number of pyknotic nuclei of tubular cells per field. There was an increase in thickness of the glomerular basement membrane and irregular foot processes of podocytes. All these findings were ameliorated by administration of atorvastatin in group VI. In group VII, some features of renal damage were noticed indicating the failure of complete recovery. The morphometric results and statistical analysis confirmed the histological findings. It was concluded that atorvastatin protected the kidney from urografin induced injury in the presence of dehydration as a risk factor.